Microbiology lab report on Escherichia coli and Staphylococcus aureus.

Microbiology Lab ReportPractica #1BTC307LAmber AmelingmeierThursday, September 18, 2008OBJECTIVESIn this lab experiment ii diametral types of bacteria, Escherichia coli and Staphylococcus aureus, were grown singly and mixed on quad diverse types of nutrient agar-agar in order to look out the vary morphologies deep down the colonies. Resulting data was analyzed to suffer understanding of the using up of differing culture media and conditions for bacterial ontogenesis. RESULTSFour different agar types were use in this experiment. The first ( food for thought) bequeathed for proceeds of twain E. coli and S. aureus. The second agar used (MKL) inhibited the proceeds of S. aureus exclusively allowed the growth of E. coli. The third (ash tree) proceedsed growth of both bacterial species. The one-fourth (mannitol) was found to inhibit growth of E. coli (Table 1). Table 1. reading material of growth within the four types of agar. G = growth, NG = no growth. E. coliS . aureusMixed cultureNutrientGGGMKLGNGGASHGGGMannitolNGGGChanges in colour within the sampled cultures were similarly noted. No limitings occurred within the Nutrient agar. The MKL agar exhibited a color change with E. coli and the mixed culture. The agar changed from red to purple, and a evident border was present touch the colonies that exhibited growth on the dish. The ASH agar did not yield a color change. The Mannitol agar exhibited a change with S. aureus and the mixed culture, but no change with E. coli (Table 2). Table 2.

Indication of color change within the four types of agar. E. coliS. aureusMixe d cultureNutrientTransparentTransparentTrans! parentMKLRed to PurpleRedRed to PurpleASHRedRedRedMannitolRedRed to YellowRed to YellowCONCLUSIONS AND DISCUSSIONTwo noticeable changes occurred during this experiment. First, two of the twelve inoculated Petri dishes yielded no growth. In order to explain these occurrences, we moldiness first examine the differing properties of the types of agar used. Nutrient agar is enriched to allow growth of a coarse variety... If you want to get a full essay, order it on our website: OrderCustomPaper.com

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